I tried it just this week. My 8 week old had a yellow discharge in one eye, I cleaned it with warm water, but was advised to try the breastmilk. Since I couldn't get to a doctor until the next day I thought I'd try it. I cleaned it with water first then expressed milk onto a clean cotton ball and wiped her eye with that, and actually squeezed a couple of drops from it into her eye (far easier than trying to express directly into her eye). I did that about three times overnight and the next day her eye was fine, has been clear since.