No I did not use whole blood, no. Disclaimer: I work in health care.
This is right up my alley but not for others. I'll only share what I found to show you HOW LONG the hcg flows in our system before a home pregnancy test can pick it up!
I read a medical article where Emergency Room doctors do this when they need a quick result and don't want to wait to send a sample down to the lab. Then I read a thread where dozens of ladies did this for laughs, and some got BFNs others got BFPs a few days earlier than a hpt. So I had to try!
Source 1
Clinical Trial results (serum is more accurate than urine, but WB can be used, too)
The ER doctors used whole blood because they need immediate answer, but I wasn't in a hurry and I know erythrocytes settle into a clot on the bottom and serum floats into a clear liquid all on its own if you let it sit for a while.
I used a glucose test lancet on a toe. (Because if you've ever stepped on something, you might remember it keeps going and going and going). I held a tiny plastic container (one of the tiny plastic pockets where my prenatal vitamins come) until it stopped on its own. I was surprised how quickly a few drops added up.
I let it sit. After a couple of hours the erythrocytes settle in the bottom and clot. The serum floats into a clear liquid on top.
I dipped a strip to test it with an IC and poured the rest on top of the FRER before dipping it for 5 seconds in SMU.
At 7 DPO there was a double line, BFP but faint.
At 9 DPO it was a stronger BFP, but I was still getting BFNs with urine samples.
Today at 11 DPO it finally showed on SMU.
I didn't quite believe it since my pregnancy tests kept coming back negative or looked like evaps. I figured I caught another chemical.
Finally, it came up on an IC today.
So this process gave me an answer 4 days before it started to showed on SMU!!!
I'm only at 11 DPO so it could still end in a chemical. My definition of a BFP is bold double lines after 15 DPO and betas that keep rising!
But this did calm my nerves. I'm still puzzled at the big difference in time and lines.
https://i63.tinypic.com/2prg179.jpg
And this are my 11 DPO urine samples:
https://i68.tinypic.com/2qi3i1f.jpg
https://i63.tinypic.com/kbw3yv.jpg
https://i65.tinypic.com/34rjcea.jpg
At 7 DPO I had a very clear BFP on serum. But I washed it and rubbed it too much. I learned you can wash the strip by running water over it - without touching it.