mickellwife - I think the vit B can interfer with OPK lines. I always had really strong lines for around two days, first cycle I took b6 (b100 complex), my OPK lines never went dark. Thought I hadn't ovulated yet, but then temps stayed up and FF confirmed OV, then 9 days later I knew I definitely OVed because I got a BFP. A few other ladies in this thread reported similar effects to their OPK results after starting B6. Just wanted to let you know that you may well have an LH surge even if the test line never gets that dark.