No, it won't matter. Your temps can fluctuate during AF but will regulate after AF is gone. You will have lower temps before ov then higher temps after ov. Remember, it's not one temp by itself that's important, it's the pattern. It's easy to get one wacky temp and think all sorts of things. I've done that so many times, lol! Just focus on a pattern, it will save you a lot of stress.